Enumeration of Coliforms and E.coli

Enumeration of Coliforms and E. coli Objective The objective of this experiment was to comp ar and credit line several(prenominal) different enumeration methods, which were utilise to determine the approximate microbic populations in foods. The enumeration methods that be performed in this experiment are the spread home office, Petrifilm habitation, and spiral coat for aerobic coat count, coliform, Petrifilm violet Red Bile (VRB), and most probable heel (MPN) techniques. Materials and Methods revel refer to the FDST (MIBO) 4030 Lab Manual for the materials and methods used for this experiment1. Results skirt 1 aerophilic plate MethodColiform Forming building block (cfu/mL) Spiral Plate2.20E3 make do Plate1.80E4 Petrifilm Plate2.01E4 When plotting the data for the Aerobic plate methods, that screwing be seen in Table 1, we discovered that the results roll out from 2.20E3 dependance forming units/mL to 2.01E4 resolution forming units/mL, which i s a difference of almost 10 folds. The method with the least(prenominal) colony forming units per mL is the spiral plate method with 2.2E3, followed by the spread plate with 1.8E4, and lastly the Petrifilm plate method with the great count of 2.01E4. Table 2 Coliform Method colonization Forming Unit (cfu/mL) Solid Medium/Pour plate1.40E2 Petrifilm VRB2.00E2 MPN2.

10E2 The results for the coliform methods as seen above in Table 2 show that the colony forming units per mL do not vary as much; they only range from 1.40E2 to 2.10E2. The solid medium pour plate method has the last(a) count with 1.40E2, follow ed by the Petrifilm VRB, with 2.0E2, and las! t with the highest count, the MPN (Most seeming Number) method with 2.1E2. handling When using the aerobic plate methods, our group used the 102 dilution for the spread plate and Petrifilm plate and one C dilution for the spiral plate method due to the fact that they wildcat well into the suitable colony counting range of 25 to 250. by and by converting our colony counts into the colony forming units/mL our results show,...If you want to occur a full essay, order it on our website: OrderCustomPaper.com

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